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PHORETIX INTERNATIONAL LIMITED 1d version 3.0
1d Version 3.0, supplied by PHORETIX INTERNATIONAL LIMITED, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/1d+version+3%2E0/1d+version+3+0/pmc01243241-68-6-5
Average 90 stars, based on 1 article reviews
1d version 3.0 - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

Electrophoresis:

Article Title: Normal human lung fibroblasts differently modulate interleukin-10 and interleukin-12 production by monocytes: implications for an altered immune response in pulmonary chronic inflammation.
Article Snippet: The ability of lung fibroblasts to modulate the immune response has been evaluated by analyzing the synthesis and release of interleukin (IL)-10 and IL-12 by lipopolysaccharide (LPS)-stimulated peripheral blood monocytes exposed to pulmonary fibroblast conditioned medium (FCM).. IL-10 and IL-12 contents and gene expression were markedly modified by treatment with FCM as measured by ELISA ( 97.5 12.8% and –68 7.3% for IL-10 and IL-12, respectively), immunocytochemistry, and reverse transcriptase–polymerase chain reaction (RT-PCR).. These effects appeared to be mediated by prostaglandin E 2 (PGE 2 ) as the modified release of both cytokines was reduced by treatment with indomethacin and mimicked by addition of exogenous PGE 2.

Article Title: Preferential channeling of energy fuels toward fat rather than muscle during high free fatty acid availability in rats.
Article Snippet: The preferential channeling of different fuels to fat and changes in the transcription profile of adipose tissue and skeletal muscle are poorly understood processes involved in the pathogenesis of obesity and insulin resistance.. Carbohydrate and lipid metabolism may play relevant roles in this context.. Freely moving lean Zucker rats received 3and 24-h infusions of Intralipid (Pharmacia and Upjohn, Milan, Italy) plus heparin, or saline plus heparin, to evaluate how an increase in free fatty acids (nonesterified fatty acid [NEFA]) modulates fat tissue and skeletal muscle gene expression and thus influences fuel partitioning.

Article Title: Multiple symmetric lipomatosis may be the consequence of defective noradrenergic modulation of proliferation and differentiation of brown fat cells.
Article Snippet: Enzo Nisoli,1,2* Laura Regianini,1 Luca Briscini,1 Alessandra Bulbarelli,1 Luca Busetto,3 Alessandra Coin,3 Giuliano Enzi3 and Michele O Carruba1,2 1 Center for Study and Research on Obesity, Department of Preclinical Sciences, LITA Vialba, L. Sacco Hospital, University of Milan, Via G. B. Grassi 74, 20157 Milan, Italy 2 Istituto Auxologico Italiano, Via Spagnoletto 3, Milan 20149, Italy 3 Division of Internal Medicine, University of Padua, Via Giustiniani 2, 35128 Padua, Italy

Article Title: Interaction between human lung fibroblasts and T-lymphocytes prevents activation of CD4 + cells
Article Snippet: Bands were analyzed with the Phoretix 1D version 3.0.

Article Title: Induction of fatty acid translocase/CD36, peroxisome proliferator-activated receptor-gamma2, leptin, uncoupling proteins 2 and 3, and tumor necrosis factor-alpha gene expression in human subcutaneous fat by lipid infusion.
Article Snippet: After amplification, 10 μl of the reaction mixture was separated by electrophoresis (1.2% agarose gel in Tr i s - a c e t a t e - E D TA buffer), visualized using ethidium bromide staining and a QuickImage-D system (Camberra Packard, Milan, Italy), and densitometrically analyzed using Phoretix 1D version 3.0.

Agarose Gel Electrophoresis:

Article Title: Normal human lung fibroblasts differently modulate interleukin-10 and interleukin-12 production by monocytes: implications for an altered immune response in pulmonary chronic inflammation.
Article Snippet: The ability of lung fibroblasts to modulate the immune response has been evaluated by analyzing the synthesis and release of interleukin (IL)-10 and IL-12 by lipopolysaccharide (LPS)-stimulated peripheral blood monocytes exposed to pulmonary fibroblast conditioned medium (FCM).. IL-10 and IL-12 contents and gene expression were markedly modified by treatment with FCM as measured by ELISA ( 97.5 12.8% and –68 7.3% for IL-10 and IL-12, respectively), immunocytochemistry, and reverse transcriptase–polymerase chain reaction (RT-PCR).. These effects appeared to be mediated by prostaglandin E 2 (PGE 2 ) as the modified release of both cytokines was reduced by treatment with indomethacin and mimicked by addition of exogenous PGE 2.

Article Title: Preferential channeling of energy fuels toward fat rather than muscle during high free fatty acid availability in rats.
Article Snippet: The preferential channeling of different fuels to fat and changes in the transcription profile of adipose tissue and skeletal muscle are poorly understood processes involved in the pathogenesis of obesity and insulin resistance.. Carbohydrate and lipid metabolism may play relevant roles in this context.. Freely moving lean Zucker rats received 3and 24-h infusions of Intralipid (Pharmacia and Upjohn, Milan, Italy) plus heparin, or saline plus heparin, to evaluate how an increase in free fatty acids (nonesterified fatty acid [NEFA]) modulates fat tissue and skeletal muscle gene expression and thus influences fuel partitioning.

Article Title: Multiple symmetric lipomatosis may be the consequence of defective noradrenergic modulation of proliferation and differentiation of brown fat cells.
Article Snippet: Enzo Nisoli,1,2* Laura Regianini,1 Luca Briscini,1 Alessandra Bulbarelli,1 Luca Busetto,3 Alessandra Coin,3 Giuliano Enzi3 and Michele O Carruba1,2 1 Center for Study and Research on Obesity, Department of Preclinical Sciences, LITA Vialba, L. Sacco Hospital, University of Milan, Via G. B. Grassi 74, 20157 Milan, Italy 2 Istituto Auxologico Italiano, Via Spagnoletto 3, Milan 20149, Italy 3 Division of Internal Medicine, University of Padua, Via Giustiniani 2, 35128 Padua, Italy

Article Title: Interaction between human lung fibroblasts and T-lymphocytes prevents activation of CD4 + cells
Article Snippet: Bands were analyzed with the Phoretix 1D version 3.0.

Article Title: Induction of fatty acid translocase/CD36, peroxisome proliferator-activated receptor-gamma2, leptin, uncoupling proteins 2 and 3, and tumor necrosis factor-alpha gene expression in human subcutaneous fat by lipid infusion.
Article Snippet: After amplification, 10 μl of the reaction mixture was separated by electrophoresis (1.2% agarose gel in Tr i s - a c e t a t e - E D TA buffer), visualized using ethidium bromide staining and a QuickImage-D system (Camberra Packard, Milan, Italy), and densitometrically analyzed using Phoretix 1D version 3.0.

Amplification:

Article Title: Normal human lung fibroblasts differently modulate interleukin-10 and interleukin-12 production by monocytes: implications for an altered immune response in pulmonary chronic inflammation.
Article Snippet: The ability of lung fibroblasts to modulate the immune response has been evaluated by analyzing the synthesis and release of interleukin (IL)-10 and IL-12 by lipopolysaccharide (LPS)-stimulated peripheral blood monocytes exposed to pulmonary fibroblast conditioned medium (FCM).. IL-10 and IL-12 contents and gene expression were markedly modified by treatment with FCM as measured by ELISA ( 97.5 12.8% and –68 7.3% for IL-10 and IL-12, respectively), immunocytochemistry, and reverse transcriptase–polymerase chain reaction (RT-PCR).. These effects appeared to be mediated by prostaglandin E 2 (PGE 2 ) as the modified release of both cytokines was reduced by treatment with indomethacin and mimicked by addition of exogenous PGE 2.

Article Title: Preferential channeling of energy fuels toward fat rather than muscle during high free fatty acid availability in rats.
Article Snippet: The preferential channeling of different fuels to fat and changes in the transcription profile of adipose tissue and skeletal muscle are poorly understood processes involved in the pathogenesis of obesity and insulin resistance.. Carbohydrate and lipid metabolism may play relevant roles in this context.. Freely moving lean Zucker rats received 3and 24-h infusions of Intralipid (Pharmacia and Upjohn, Milan, Italy) plus heparin, or saline plus heparin, to evaluate how an increase in free fatty acids (nonesterified fatty acid [NEFA]) modulates fat tissue and skeletal muscle gene expression and thus influences fuel partitioning.

Article Title: Multiple symmetric lipomatosis may be the consequence of defective noradrenergic modulation of proliferation and differentiation of brown fat cells.
Article Snippet: Enzo Nisoli,1,2* Laura Regianini,1 Luca Briscini,1 Alessandra Bulbarelli,1 Luca Busetto,3 Alessandra Coin,3 Giuliano Enzi3 and Michele O Carruba1,2 1 Center for Study and Research on Obesity, Department of Preclinical Sciences, LITA Vialba, L. Sacco Hospital, University of Milan, Via G. B. Grassi 74, 20157 Milan, Italy 2 Istituto Auxologico Italiano, Via Spagnoletto 3, Milan 20149, Italy 3 Division of Internal Medicine, University of Padua, Via Giustiniani 2, 35128 Padua, Italy

Article Title: Interaction between human lung fibroblasts and T-lymphocytes prevents activation of CD4 + cells
Article Snippet: Bands were analyzed with the Phoretix 1D version 3.0.

Article Title: Induction of fatty acid translocase/CD36, peroxisome proliferator-activated receptor-gamma2, leptin, uncoupling proteins 2 and 3, and tumor necrosis factor-alpha gene expression in human subcutaneous fat by lipid infusion.
Article Snippet: After amplification, 10 μl of the reaction mixture was separated by electrophoresis (1.2% agarose gel in Tr i s - a c e t a t e - E D TA buffer), visualized using ethidium bromide staining and a QuickImage-D system (Camberra Packard, Milan, Italy), and densitometrically analyzed using Phoretix 1D version 3.0.

Staining:

Article Title: Normal human lung fibroblasts differently modulate interleukin-10 and interleukin-12 production by monocytes: implications for an altered immune response in pulmonary chronic inflammation.
Article Snippet: The ability of lung fibroblasts to modulate the immune response has been evaluated by analyzing the synthesis and release of interleukin (IL)-10 and IL-12 by lipopolysaccharide (LPS)-stimulated peripheral blood monocytes exposed to pulmonary fibroblast conditioned medium (FCM).. IL-10 and IL-12 contents and gene expression were markedly modified by treatment with FCM as measured by ELISA ( 97.5 12.8% and –68 7.3% for IL-10 and IL-12, respectively), immunocytochemistry, and reverse transcriptase–polymerase chain reaction (RT-PCR).. These effects appeared to be mediated by prostaglandin E 2 (PGE 2 ) as the modified release of both cytokines was reduced by treatment with indomethacin and mimicked by addition of exogenous PGE 2.

Article Title: Preferential channeling of energy fuels toward fat rather than muscle during high free fatty acid availability in rats.
Article Snippet: The preferential channeling of different fuels to fat and changes in the transcription profile of adipose tissue and skeletal muscle are poorly understood processes involved in the pathogenesis of obesity and insulin resistance.. Carbohydrate and lipid metabolism may play relevant roles in this context.. Freely moving lean Zucker rats received 3and 24-h infusions of Intralipid (Pharmacia and Upjohn, Milan, Italy) plus heparin, or saline plus heparin, to evaluate how an increase in free fatty acids (nonesterified fatty acid [NEFA]) modulates fat tissue and skeletal muscle gene expression and thus influences fuel partitioning.

Article Title: Multiple symmetric lipomatosis may be the consequence of defective noradrenergic modulation of proliferation and differentiation of brown fat cells.
Article Snippet: Enzo Nisoli,1,2* Laura Regianini,1 Luca Briscini,1 Alessandra Bulbarelli,1 Luca Busetto,3 Alessandra Coin,3 Giuliano Enzi3 and Michele O Carruba1,2 1 Center for Study and Research on Obesity, Department of Preclinical Sciences, LITA Vialba, L. Sacco Hospital, University of Milan, Via G. B. Grassi 74, 20157 Milan, Italy 2 Istituto Auxologico Italiano, Via Spagnoletto 3, Milan 20149, Italy 3 Division of Internal Medicine, University of Padua, Via Giustiniani 2, 35128 Padua, Italy

Article Title: Interaction between human lung fibroblasts and T-lymphocytes prevents activation of CD4 + cells
Article Snippet: Bands were analyzed with the Phoretix 1D version 3.0.

Article Title: Induction of fatty acid translocase/CD36, peroxisome proliferator-activated receptor-gamma2, leptin, uncoupling proteins 2 and 3, and tumor necrosis factor-alpha gene expression in human subcutaneous fat by lipid infusion.
Article Snippet: After amplification, 10 μl of the reaction mixture was separated by electrophoresis (1.2% agarose gel in Tr i s - a c e t a t e - E D TA buffer), visualized using ethidium bromide staining and a QuickImage-D system (Camberra Packard, Milan, Italy), and densitometrically analyzed using Phoretix 1D version 3.0.



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